Pure source 2 filter system12/17/2023 ![]() Sheep milk fermented using Lactobacillus could be considered a novel source of antioxidative and antimicrobial proteins.īulet P, Cociancich S, Dimarcq JL, Lambert J, Reichhart JM, Hoffmann D, Hetru C, Hoffmann JA (1991) Insect immunity. BIOPEP, a database for antioxidative and antimicrobial peptides, validated the antioxidative & antimicrobial activities of several peptides in sheep's milk that has been fermented. On electrophoresis on a 2-D gel, 6 peptides were identified using RP-LC/MS. The molecular weight of the protein was estimated using SDS-PAGE. typhimurium (13 mm) and 3 kDa retentate against E. coli (13.3 mm) was greater than 3 and 10 kDa retentate against S. Antimicrobial activity of 10 kDa retentate against B. The hydroxyl free radical scavenging activity was greatest in 10 kDa retentate and superoxide free radical scavenging activity was observed in 3 kDa permeate (34.7, 43.4, and 34.6%, respectively). Fermented sheep milk fractions of 3 & 10 kDa were analysed for antioxidative and antimicrobial activity, and the 10 kDa permeate showed the highest ABTS assay. However, inoculation of culture at a level of 2.5% and 48 h fermentation give the highest proteolysis activities. It was inferred that antioxidative and antimicrobial activities increased with increasing incubation time, and antioxidative properties (ABTS assay, superoxide free radical & hydroxyl free radical scavenging activity were 34.5, 34.7, and 29.2% respectively) and antimicrobial properties against Escherichia coli, S. Failure to do so may void warranty.This study aims to identify antioxidant and antimicrobial peptides from sheep milk produced using Lactobacillus plantarum (KG元A). Important: It is essential that operation, maintenance and filter replacement be carried out in accordance with manufacture's recommended procedures and guidelines for this product to perform as advertised. When to change a filter: The filter cartridge should be replaced when the change filter light comes on, or every 6 months.
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